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Given my views on play, you won’t be surprised to learn that I asked the presenters about whether play experiences could qualify as the kinds of responsive, positive, engaged, and empowering interactions they described. I was attending professional development aimed at helping library staff support families from refugee backgrounds, and we were discussing the role that libraries and childcare staff (mostly the latter, based on the mix of the group) can play in helping often highly traumatised communities. A small increase in risk of harm, replicated many times on a massive scale, means harm is certainly occurring. As COVID-19 lockdowns unfolded around the world, and sales of games, puzzles, toys, and other playful artforms spiked, a friend asked me if I felt a sense of validation. And worst of all, a combination of the two, where other players essentially become supporting cast for a scripted victory that is neither earned, nor fun, nor remotely playful – a psychodrama rather than a game, toying with their victims rather than playing with them. Controlling bullies who insist everyone play their way, forcing people to make deals they don’t want and to keep playing even when they aren’t having fun.
Emotional distress is also very common among family members. In this article, we explore how families can support the path to sobriety, the various dynamics, and the resources available for both the individual and their loved ones. Recovery from addiction is a journey that would be helped by involving the entire family unit. Make a one-time donation or a recurring donation to Café RE here. By Johanna C, 3 months sober (at the time of writing), most recent sober date August 18th, 2023. Through this exercise, I was able to reflect on the tape that illustrates my life as an anxiety-ridden, unpredictable and obsessive drinker.

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Briefly, worms were well fed for at least two generations, and gravid adults were bleached with hypochlorite and sodium hydroxide. L1 starvation assay was adapted from a previously described protocol (3). Worms strains were grown and maintained at 20 °C as described (29). This result is consistent with the observation that miR-71 is specifically required for the starvation-induced stress response (Fig. S5). For example, we observed a robust retarded mutant phenotype in the vulval lineage but did not see obvious defects in seam cell differentiation or alae formation. Our data provide the experimental evidence that two components of the InsR pathway are likely direct targets of miR-71 in its role in a specific physiological process, L1 diapause (see a model in Fig. S5).

• Mental health recovery doesn’t happen in isolation, it affects and involves others too.
• On the other hand, the role of a particular miRNA (miR-71) is executed by repressing the expression of many genes in multiple pathways.
• Have there been any changes to your account details that you didn’t make (email address, Activision ID, etc.)?
• But from a colour management and overall interpretation viewpoint, I don’t know what to do with this image, and to be honest, I like the hues you’ve achieved in the sky.
• Instead, many specific physiological functions, such as the starvation-induced stress response, are regulated by a miRNA-target network, often involving multiple miRNAs and a large number of their targets.

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• These therapeutic groups are not unstructured sharing spaces, but guided experiences designed to support emotional regulation, trauma resolution, and nervous system healing.
• These results suggest that miR-71 regulates the expression of unc-31 and age-1 through their 3′UTRs.
• We found that the reduced survival rate of ain-1 was suppressed by either reduction of age-1 function or loss of unc-31 function (Fig. 1 B and C), suggesting that a significant portion of the overall miRNA functions in L1 diapause is upstream of, or in parallel to, the InsR pathway.
• On 12 February 2020, they announced that lead singer Biesler would be leaving the band.
• Eastern time.
• Fun seems self-evident, but I would just gently remind readers that fun is non-trivial; is in fact the active face of joy, as beauty is the passive.

What is the importance of setting boundaries in family recovery?

Furthermore, worms that are long-lived due to dietary restriction or decreased mitochondrial respiratory rates are short-lived during L1 diapause, suggesting that the mechanisms controlling L1 starvation survival are different at least in some aspects from those controlling aging (3). We provide evidence that miRNA miR-71 is not required for the animals’ entry into L1 diapause, but plays a critical role in long-term survival by repressing the expression of insulin receptor/PI3K pathway genes and genes acting downstream or in parallel to the pathway. We need to be promoting better-quality games that do more to help people have fun, and we need to be modelling and making room for healthy play that promotes pro-social behaviour. That means running programs, preferably having staff who can teach the games, having services that help people find others who can teach and/or are interested in playing, and establishing norms for play in the library space in the hopes of (a) offering that safe, and potentially healing, interaction; (b) having those norms spread.
(D) A representative chart of the L1 starvation survival rates of different miRNA mutants. However, it remains unclear how, and to what extent, miRNAs coordinate animal survival and development in response to stresses. Unlike dauer diapause, L1 diapause is not accompanied by life cycle changes and has not been shown to require certain signaling pathways that control the formation of dauer diapause such as TGF-β signaling (daf-1, daf-7) and nuclear hormone receptor (daf-12) (2, 3). When late, first larval stage (L1) worms sense unfavorable conditions, they enter an alternative and long-lived larval stage called dauer larvae (or dauer diapause). The nematode Caenorhabditis elegans responds to starvation by entering developmental arrest at multiple stages of its life cycle (1).
This feels like one of those posts where if I had more time I could make it shorter… but I don’t. Fun seems self-evident, but I would just gently remind readers that fun is non-trivial; is in fact the active face of joy, as beauty is the passive. As above, play without consent, treating others as toys without independent will rather than as fellow players, is oppression and abuse. In fact, one of the great, almost pre-verbal lessons of play is precisely that the magic circle of play exists only with, is in large part comprised of, the willing participation of everyone in and around it. That curation isn’t only about having collections available for loan or in the library space; it’s also about making actual play experiences available.
However, we found that the reporter transgene with the lin-42 3′UTR was significantly repressed in wild-type worms, but derepressed in the mir-71(lf) worms (Fig. 4 H and I). In starved L1 worms, we detected only a slight increase in the mRNA level of hbl-1 in mir-71 mutants compared with that in wild type (∼10%), which may not be biologically significant. These results indicate that miR-71 plays a significant role in larval development of animals recovering from L1 diapause and likely does so by regulating the expression of components of the insulin receptor/DAF-16 pathway, as well as factors acting downstream, or in parallel to, DAF-16. If this were true, the starved mir-71(lf); daf-16(lf) double-mutant worms should show a slow growth phenotype similar to that of daf-16(lf) worms, but no specific VPC timing defect.

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Waking up not knowing what you did last night and that when we promise we’ll never drink again, it’s quite fine when we reach for the wine and wine glass the next weekend when happy hour hits. And for those of us who have taken a step into the other side, regardless of sobriety time, see it. A mechanism through which we can move through our addiction and keep saying YES to an AF life.
I’m fighting for a reimbursement, but I’m posting this so you don’t lose the resources I did.” Support isn’t just unhelpful; they aren’t even in the same reality as the players. “To the people calling me an ‘EA Spokesman’—take a look at my latest interaction with them. This study is supported by National Institutes of Health Grant GM47869 (to M.H.).

Everyone Can Play A Part In The Recovery Journey

MiR-71 regulates vulval cell division during recovery of starved L1 worms. We thus asked whether miR-71 was required for the reinitiation of developmental programs during the recovery phase after L1 starvation. revery play These results indicate that miR-71 is not essential for arresting seam cell or M-cell divisions during L1 diapause, suggesting that miR-71 function is distinct from DAF-16 function. DAF-16 (the FOXO homolog in C. elegans) has been shown to play an important role in cell cycle arrest and developmental progression partly by promoting cki-1 expression in some somatic cells during L1 arrest (2). S1A indicated a dominant role of intestinal miRNAs in regulating L1 starvation survival.